Nanomechanics of Diaminopurine-Substituted DNA
نویسندگان
چکیده
منابع مشابه
Footprinting of echinomycin and actinomycin D on DNA molecules asymmetrically substituted with inosine and/or 2,6-diaminopurine.
In order to clarify the role of the purine 2-amino group in the recognition of DNA by small molecules we have examined the binding of actinomycin D and echinomycin to artificial DNA molecules asymmetrically substituted with inosine and/or 2,6-diaminopurine (DAP) in one of the complementary strands. These DNAs, prepared by a method based upon PCR, present various potential sites for antibiotic b...
متن کاملDNA nanomechanics: how proteins deform the double helix.
It is a standard exercise in mechanical engineering to infer the external forces and torques on a body from a given static shape and known elastic properties. Here we apply this kind of analysis to distorted double-helical DNA in complexes with proteins: We extract the local mean forces and torques acting on each base pair of bound DNA from high-resolution complex structures. Our analysis relie...
متن کاملNanomechanics of carbon nanotubes.
Some of the most important potential applications of carbon nanotubes are related to their mechanical properties. Stiff sp2 bonds result in a Young's modulus close to that of diamond, while the relatively weak van der Waals interaction between the graphitic shells acts as a form of lubrication. Previous characterization of the mechanical properties of nanotubes includes a rich variety of experi...
متن کاملNanomechanics of HaloTag tethers.
The active site of the Haloalkane Dehydrogenase (HaloTag) enzyme can be covalently attached to a chloroalkane ligand providing a mechanically strong tether, resistant to large pulling forces. Here we demonstrate the covalent tethering of protein L and I27 polyproteins between an atomic force microscopy (AFM) cantilever and a glass surface using HaloTag anchoring at one end and thiol chemistry a...
متن کاملQuantum Nanomechanics
Quantum Nanomechanics is the emerging field which pertains to the mechanical behavior of nanoscale systems in the quantum domain. Unlike the conventional studies of vibration of molecules and phonons in solids, quantum nanomechanics is defined as the quantum behavior of the entire mechanical structure, including all of its constituents—the atoms, the molecules, the ions, the electrons as well a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2019
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2019.01.027